Integrated analysis of mRNA and miRNA profiles revealed the role of miR-193 and miR-210 as potential regulatory biomarkers in different molecular subtypes of breast cancer

Abstract Background Breast cancer is the most frequently diagnosed malignancy among women. However, role of microRNA (miRNA) expression in breast progression not fully understood. In this study we examined predictive interactions between differentially expressed miRNAs and mRNAs cell lines representative common molecular subtypes. Integrative bioinformatics analysis identified miR-193 miR-210 as potential regulatory biomarkers mRNA cancer. Several recent studies have investigated these a broad range tumors, but mechanism their involvement has previously been investigated. Methods The miRNA-mRNA were by parallel miRNA target prediction programs. profiles from luminal (MCF-7, MCF-7/AZ T47D), HER2 (BT20 SK-BR3) triple negative subtypes (Hs578T e MDA-MB-231) could be clearly separated unsupervised using HB4A line control. data TCGA patients grouped according to then used validate findings. Expression was transient silencing assays MCF7, BT20 MDA-MB-231 lines. Functional included, xCELLigence system, ApoTox-Glo triplex assay, flow cytometry transwell inserts performed determine proliferation, cytotoxicity, apoptosis, migration invasion, respectively. Results evident effects associated with proliferation after subtype MDA-MB-231. Using silico algorithms, TNFRSF10 one regulated downstream targets for both miRNAs. TNFRSF10C TNFRSF10D inversely correlated levels miR210 patients, Other genes whose also CCND1 , known mediator on invasion metastasis, tumor suppressor gene RUNX3 . Conclusions summary, our findings identify different suggest that may specific proliferation. Our results highlight important new serve promising therapeutic pathways aggressive cancers